重组人源胶原蛋白Recombinant human collagen

产品包装规格：

种属：             Human Cells

表达系统：      Prokaryotic expression system、Eukaryotic expression system 

标签：             not have

同用名：         重组人源化胶原蛋白、Recombinant human collagen、胶原蛋白

分子量：          13.10 KDa

纯度：             Greater than 95% as determined by Tris-Bis PAGE.

储存条件：      Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 100mM NaCl, pH7.5.

备注：             Always centrifuge tubes before opening.Do not mix by vortex or pipetting.

                       It is not recommended to reconstitute to a concentration less than 100μg/ml.

                       Dissolve the lyophilized protein in distilled water.

                       Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

储存时间：      Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.

                       Reconstituted protein solution can be stored at 2-8°C for 2-7 days.

                       Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

运输：             The product is shipped at ambient temperature.

                       Upon receipt, store it immediately at the temperature listed below.

背景：            重组人源化胶原蛋白是基于人体皮肤Ⅲ型胶原蛋白的原始基因序列，优化选择其中水溶性强、生物活性高的部分进行密码子优化和拼接重组，得到了全新的重组人源化Ⅲ型胶原蛋白序列，利用生物发酵技术实现可规模化生产，通过实验证实此胶原蛋白表达量大，水溶性好，生物活性高，其性能优于人天然胶原蛋白，在生物医用材料、美容化妆品、食品保健等领域有着广大的应用前景。

胶原蛋白种类较多，常见类型为Ⅰ型、Ⅱ型、Ⅲ型、Ⅴ型和Ⅺ型。

在正常皮肤组织中，胶原主要以I、III型胶原纤维的形式存在。

I型胶原和Ⅲ型胶原与皮肤损伤修复过程和修复质量紧密相关。正常胎儿皮肤Ⅲ型胶原占 60%，随着生长发育，Ⅲ型胶原不断减少，I型胶原不断增加。成人皮肤胶原组成为 I型占80%，Ⅲ型占20%。胎儿无瘢痕愈合机制是由于胎儿较强的Ⅲ型胶原的合成能力。

重组人源化胶原蛋白基础结构跟人体一模一样的，同时在此基础上进行优化，提高胶原蛋白的亲水性以及活性。含有大量的的亲水性基因，具有良好的成膜性，保持皮肤角质层水分。同时,它的趋向性引导作用可引导上皮细胞快速进入受损部位，有效提高皮肤再生速度，缩短创伤愈合时间，从而恢复皮肤屏障功能。

是基于人Ⅲ型胶原蛋白的原始基因序列，选择水溶性强、生物活性高的部分进行密码子优化和重组，采用大肠杆菌表达系统，利用生物发酵技术实现可规模化生产的一种胶原蛋白，具有与人100%同源，水溶性强、生物活性高、透皮吸收率高等特点。拥有独特的164.88°三螺旋柔性曲度，这种结构可以显著增强胶原蛋白对细胞的粘附力。

重组人源胶原蛋白是应用基因工程技术合成的一种高纯度胶原蛋白，具有出色的生物相容性、纯度和稳定性。它在医疗、美容和抗衰老领域有着广泛应用，可以促进组织修复和再生，改善皮肤质量，减少皱纹，恢复年轻外貌。本文将重组人源胶原蛋白的应用展开讨论，并探讨了其发展面临的挑战和未来的发展前景。

Recombinant human collagen is based on the original gene sequence of human skin type III collagen, and the codon optimization and splicing of the parts with strong water solubility and high biological activity are optimized to obtain a brand new recombinant human type III collagen sequence. Biological fermentation technology is used to achieve large-scale production. Experiments have confirmed that this collagen has high expression and good water solubility. With high biological activity, its properties are better than natural human collagen, and it has broad application prospects in biomedical materials, beauty cosmetics, food health and other fields.

In normal skin tissue, collagen mainly exists in the form of type I and III collagen fibers.

Type I collagen and type III collagen are closely related to the process and quality of skin damage repair. Type III collagen accounts for 60% of normal fetal skin. With the growth and development, type III collagen is continuously reduced, and type I collagen is continuously increased. The collagen composition of adult skin is type I (80%) and Type III (20%). The mechanism of fetal scarless healing is due to the fetal's strong ability to synthesize type III collagen.

The basic structure of recombinant human collagen is exactly the same as that of human body, and on this basis, it is optimized to improve the hydrophilicity and activity of collagen. Contains a large number of hydrophilic genes, has a good film formation, keep the skin stratum corneum water. At the same time, its directional guiding effect can guide epithelial cells to enter the damaged site quickly, effectively improve the skin regeneration rate, shorten the wound healing time, and thus restore the skin barrier function.

Based on the original gene sequence of human type III collagen, codon optimization and recombination were performed on the parts with strong water solubility and high biological activity. A collagen protein with 100% homology with human, strong water solubility, high biological activity and high transdermal absorption rate was realized by biological fermentation technology using the expression system of Escherichia coli. With a unique 164.88° triple helix flexure, this structure significantly enhances the adhesion of collagen to cells.

Recombinant human collagen is a kind of high purity collagen synthesized by genetic engineering technology, which has excellent biocompatibility, purity and stability. It has a wide range of applications in medical, cosmetic and anti-aging fields to promote tissue repair and regeneration, improve skin quality, reduce wrinkles and restore a youthful appearance. In this paper, the application of recombinant human collagen was discussed, and the challenges and prospects of its development were discussed.

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