葡萄糖氧化酶（Glucose oxidase, GOD）

产品包装规格：

种属：             黑曲霉（Aspergillus niger）和青霉（Penicillium） 

表达系统：      Prokaryotic expression system、Eukaryotic expression system 

标签：           N-His

同用名：          β-D-Glucose:oxygen 1-oxidoreductase; G.Od.;GOx;β-D-葡萄糖：氧1-氧化还原酶

分子量：         65.5 KDa

纯度：             lyophilized powder, >180 U/mg

储存条件：     This enzyme is soluble (1.0 mg/mL) in 50 mM sodium acetate buffer, pH 5.1,yielding a clear solution.

备注：            This product is for R&D use only, not for drug,household, or other uses.
                       Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

储存时间：     Dry and store at -20°C for at least 1 year.

运输：           Ice pack transport

背景：          葡萄糖氧化酶（Glucose Oxidase, EC NO. 1.1.3.4），来源于黑曲霉 （Aspergillus niger），是一种由2个相同亚基构成的二聚体，每个亚基分子量约80kDa。每个亚基包含一个黄素腺嘌呤二核苷酸（FAD）和一个铁离子。葡萄糖氧化酶是一个糖蛋白，包含~16%中性糖和2%氨基糖。同时含有3个半胱氨酸残基和8个N-连接糖基化用的潜在位点。

葡萄糖氧化酶能够不同程度的氧化D-己醛醣、单脱氧-D 葡萄糖和甲基 D-葡萄糖。在pH 4-7范围内有活性，最佳pH是5.5。特异性识别B-D-葡萄糖，KM值为33~110mM。葡萄糖氧化酶不需要任何激活剂，但活性被Ag+、Hg2+、Cu2+、醋酸苯汞和对氯汞苯甲酸（PCMB）抑制，不会被非金属SH试剂：N-乙基马来酰亚胺、碘乙酸和碘乙酰胺抑制。

葡萄糖氧化酶能用于溶液中酶法定量D-葡萄糖。由于葡萄糖氧化酶氧化B-D-葡萄糖生成D-葡萄糖内酯和H202，因此，辣根过氧化物酶（HRP）通常用作葡萄糖测定中的一种偶联酶。本品广泛用在视频和医药工业，以及用作葡萄糖生物传感器中的一种主要成分。

Glucose Oxidase (EC NO. 1.1.3.4), derived from Aspergillus niger, is a dimer composed of two identical subunits, each of which has a molecular weight of about 80kDa. Glucose Oxidase (EC NO. 1.1.3.4), derived from Aspergillus niger, is a dimer composed of two identical subunits, each of which has a molecular weight of about 80kDa. Each subunit contains a flavin adenine dinucleotide (FAD) and an iron ion. Glucose oxidase is a glycoprotein containing ~16% neutral sugars and 2% amino sugars. It also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.

Glucose oxidase can oxidize D-hexalose, monodeoxy-D-glucose and methyl-D-glucose to varying degrees. It is active in the pH 4-7 range, with an optimal pH of 5.5. Specific recognition of B-D-glucose, KM value of 33~110mM. Glucose oxidase does not require any activator, but its activity is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercurbenzoic acid (PCMB), and is not inhibited by non-metallic SH reagents: N-ethyl maleimide, iodoacetic acid, and iodoacetamide.

Glucose oxidase can be used for enzymatic quantification of D-glucose in solution. Because glucose oxidase oxidizes B-D-glucose to produce D-glucolactone and H202, horseradish peroxidase (HRP) is commonly used as a coupling enzyme in glucose assay. This product is widely used in the video and pharmaceutical industries, as well as as a major component in glucose biosensors.

样品信息：

所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

技术咨询电话: 13522791889

官方网站: http://www.aiprotein-taiji.com

邮箱: machangxing@pku.org.cn

地址: 中国（四川）自由贸易试验区成都市天府新区兴隆街道湖畔路东段733号